5 Tips about flow cytometer You Can Use Today
5 Tips about flow cytometer You Can Use Today
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Facts-enriched characterization of mobile populations. Morphological info from photos can insert towards the richness of flow cytometry information. As an example, the figure reveals an or else common apoptosis assay working with Annexin V and PI, adding mobile imaging to characterize cells in Each and every population to reveal morphologically distinctive attributes. These insights could not are received from multiplex staining alone.
No matter whether you’re a newbie or an expert, discover our sources to progress your exploration and experience in flow cytometry.
Blend acoustic focusing know-how with higher-parameter analysis for flexible spectral unmixing and standard workflows.
Find out new insights into your experimental info by visualizing populations using mixed fluorescence and morphology details. Our software program interface wizards empower downsampling, concatenation (merged information) and dimensionality equipment that are created to the software for an intuitive, streamlined details Examination expertise.
"A picture isn't really very value 1000 dots, nevertheless it genuinely helps to see photos. It is great to see visuals that correlate with staining. For instance, AnnexinV-beneficial cells which might be morphologically different than healthful cells, or CD14+ monocytes which have been larger and much more textured than CD3+/CD4+/CD14- lymphocytes.
Listed here, a highly trained consumer has gated singlets confidently. Right after evaluating the handbook singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate consists of much more than four% aggregates.
Optimizing gating tactic. Even sturdy manual singlet gating is error-susceptible and stays a subjective choice issue in Nearly all flow cytometry assays. Imaging can be utilized to verify and change gates to incorporate only single cells of interest.
Our scientists will structure panels for yourself as persistently you would like—cost-free, no item acquire necessary.
The Attune NxT cytometer could be the trusted workhorse instrument your lab can rely on for steady and trusted efficiency. The Attune CytPix product incorporates a superior-velocity brightfield digicam and AI-pushed impression Examination software, with latest upgrades which includes device learning equipment and an intuitive consumer interface, enabling Improved info precision, accuracy, and a lot quicker insights into sample biology.
Probably most significantly, these situations incorporate cells of Obviously distinct phenotypes which could lead on to incorrect conclusions about double positive events (specifically in exceptional populations).
Sound waves permit exact cell alignment, resulting in exceptional details quality and ~10X more rapidly sample flow prices.
We Formerly demonstrated the power of imaging Automobile-T/Ramos mobile interactions. Permit’s evaluate just the population of greatest desire, here the double constructive activities, To find out more. We are able to now use extended morphological parameters (below, circularity and skewness of intensity) to even further examine the functions of these populations and refine gating on these functions, rising details robustness.
Leverage the power of Highly developed know-how and innovation to speed up your discoveries. Have confidence in while in the Attune Flow Cytometer spouse and children to deliver the reliability, speed, and accuracy that established your perform aside.
Adaptable payment approach from Agilent Fiscal Solutions let you receive the vital instrumentation you will need for fast venture, or to grow your lab.
At the center of every Attune Flow Cytometer lies our groundbreaking acoustic concentrating technological know-how, which utilizes sound waves to meticulously align cells in the flow cell. This progressive strategy surpasses standard hydrodynamic solutions, leading to more quickly processing speeds, exceptional knowledge excellent, and amplified sample throughput.
As the mobile line had Formerly been analyzed to the Attune CytPix Flow Cytometer, the researchers went back to the photographs and ended up capable to document the microbial an infection no less than five times before.
The Attune CytPix Flow Cytometer makes it possible for end users to visualise samples through acquisition, validate the precision of gating techniques, and assist benefits with dependable documentation for publication reasons.
Here, we exhibit that by gating strategies utilizing graphic-dependent quantitative parameters can distinguish interacting cells from coincident activities to additional precisely analyze interacting cells.